Ensho Saisei
Online ISSN : 1880-5795
Print ISSN : 1346-8022
ISSN-L : 1346-8022
Original Article
A method for gene transfer, single isolation and in vitro assay for neural stem cells
Masanori SakaguchiKazunobu SawamotoTakuya ShimazakiToshio KitamuraAkira ShibuyaHideyuki Okano
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2005 Volume 25 Issue 1 Pages 50-54


The neurosphere method is widely used to examine self-renewal activity and the multipotency of neural stem cells (NSCs). We developed an efficient method for introducing a transgene into neurospheres using a retroviral vector and isolating a transgenic subpopulation of NSCs labeled with green fluorescent protein (GFP) using fluorescence-activated cell sorting, enabling these cells to be used in subsequent biological assays. This method is expected to be useful for studying the function of genes in NSCs.

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© 2005 by The Japanese Society of Inflammation and Regeneration
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