Abstract
An increase of the urinary prostagrandin excretion from the patient with Bartter's syndrome has been reported in recent years far from the view point of the quantitative separation of PGs. Studies on profiling analysis of the urinary PGs using new HPLC separation method were executed in our laboratories. A combination system of the HPLC method using a reversed phase column with an extraction method by use of Kiesserguhr column was recommended for their profiling analysis as described in this paper.
During the untreated period with respect to a patient of this syndrome, 6-keto (6k) PGF1α as a stable metabolite from PGI2 and PGE2 in the urine were found to increase markedly, when compared with the other PGs, while they were significantly reduced after the indomethacin therapy. However, those PGs less than several ng level were not detected by the present method. This simple and rapid HPLC method seems to be not only most useful for the profiling analysis of PGs in urine, but also applicable to those in other biological samples such as blood and tissues providing that the sensitivity in this separation method would be increased.
