Abstract
Among a number of inflammations in experimental animals we selected air-pouch inflammation induced in the subcutaneous tissues on the dorsum of rats as the most suitable type in analysing mechanisms of the inflammatory processes involving acute exudative phase but aslo chronic prolifrative phase of the inflammation. About 8 ml of air is injected on the first day and then various phlogistic stimuli are applied in the air sac already formed. Carrageenin solution, zymosan suspension in carboxymethyl cellulose (CMC) solution and azobenzenearsonate-conjugated acetylbovine serum albumin solution in CMC solution of pre-sensitized animals have been studied as th phlogistic stimuli.
Vascular permeability can be measured with the aid of radioiodinated human serum albumin as a tracer, which is injected intravenously and then leaked into the fluid in the air pouch. Infiltration of leucocyte is measurable by taking the fluid in the locus of air-pouch inflammation. The pouch fluid canbe used as a sample for a variety of chemical analyses. Proliferative processes are also measurable by taking granulation tissues as a capsule growing around the air pouch. Minced granulation tissues are suitable in investigating incorporation of various labeled precursors into macromolecular components of the tissues.
