Inhibitory effect of CCA on immunoglobulin-production induced by B151-TRF2

Abstract

Lobenzarit disodium (CCA) is an immunomodulating anti-rheumatic drug, and is reported to show inhibitory effects on B-cell activation. To further analyze the direct effects of CCA on B cells, we examined the effects of CCA on Ig-production in vitro by murine B cells stimulated with murine T cell-derived B-cell stimulation factor, B 151-TRF 2 which induces a polyclonal differentiation into Ig-secreting cells including autoantibody production.
CCA inhibited Ig-production induced by B 151-TRF 2. This result suggests that the inhibitory effect of CCA on Ig-production is one of the mechanism of CCA action on autoimmune disease, because B 151-TRF 2 is reported to be closely associated with the onset of autoimmune disease. In contrast, CCA did not inhibit Ig-production induced by LPS.
These results demonstrate that the induction process of Ig-production by B 151-TRF 2 is differ from that of Ig-production by LPS, and that the effective point of CCA is associated with this difference.