Abstract
The adherence of circulating leukocytes to the vascular endothelium is a critical step of inflammatory infiltration. In order to understand the influence of the nasal secretion on vascular endothelium, adherence of neutrophils to monolayers of endothelial cells was assayed using 51Cr-labeled leukocytes and human mucosal microvascular endothelial cells derived from inferior turbinate. Quantitative analysis of interleukin-1α, β and tumor necrosis factor-α were made in the retention fluid of the maxillary sinus of chronic sinusitis and the nasal secretion of nasal allergy using ELISA kit. The vascular endothelial cells acquired adhesiveness for neutrophils after preincubation with maxillary sinus retention fluids of chronic sinusitis. However, no adhesive effect was found in the nasal secretion of nasal allergy. Quantitative analysis revealed the presence of interleukin-1 and the absence of tumor necrosis factor-α in the retention fluids of chronic sinusitis. The adherence inducing effect was heatlabile and almost neutralized by anti-IL-1β antibody in dose dependent manner.
These findings suggest that the IL-1 in the para-nasal secretion of chronic sinusitis induce the adherence of neutrophil to vascular endothelium and further promoted leukocytes infiltrates in the paranasal sinuses, playing an important role in persistency of chronic sinusitis.
