Abstract
Superoxide production of human neutrophils was enhanced within 10 min by the treatment of interferon-γ, when phorbol 12-myristate 13-acetate (PMA) was used as a stimulant. The enhancement of superoxide production by interferon-γ occurred only under the presence of calcium, and that it was inhibited by 1- (5-isoquinolinesulfonyl) -2-methylpiperazine (H-7), an inhibitor of protein kinase-C and 8- (N, N-diethylamino) -octyl- (3, 4, 5-trimethoxy) benzoate hydrochloride (TMB-8), an intracellular calcium antagonist. Quin 2 (acetoxy-methylester) fluorescent revealed that intracellular calcium arose immediately after the addition of interferon-γ.
These data suggest that the enhancement of superoxide production by interferon-γ is closely related to protein kinase-C activation followed by the arising of intracellular calcium.
