Abstract
We found inhibitors against myeloperoxidase (MPO) release from human polymorphonuclear leukocytes (PMN) . We designated them aseanostatins (AS), because these inhibitors were extracted from an actinomycete isolated in Thailand. AS were purified by a series of column chromatography of charcoal and silicagel, and HPLC. Physico-chemical chracterization by gas liquid chromatography and GC-MS indicated that two component P 1 and P 5 as pure forms were identical with i-14: 0 (12-methyltridecanoic acid) and ante-i-15: 0 (12-methyltetradecanoic acid), respectively. AS P 1 and P 5 showed IC50 of 0.96 and 0.54 beg/ml to the MPO release, respectively. AS, however, did not inhibit β-glucuronidase release, but inhibited O-2 production a little. Moreover, those had no effects on other functions of PMN.
These findings suggest that AS may regulate MPO release or O-2 production which related to over reaction in inflammatory site.
