Determination of endothelin-1 levels and its molecular forms in human plasma, urine, cerebrospinal fluid and synovial fluid using a specific radioimmunoassay for endothelin-1

Abstract

Using a highly specific and sensitive radioimmunoassay for measurement of endothelin-1 (ET-1) and reverse-phase HPLC, the presence of ET-1-like immunoreactivity was demonstrated in human plasma, urine, cerebrospinal fluid and synovial fluid.
Determination of ET-1 levels and its molecular forms in biological fluid from several diseases should provide important clues to understanding its physiological and pathophysiological role (s) in man.