Abstract
Effect of cytokines on the production of the third component of complement (C 3) by human umbilical vein endothelial cells (HUVEC) was investigated. Interleukin-1 (IL-1), interferone-γ (IFN-γ), tumor necrosis factor-β (TNF-β), and lipopolysaccharide (LPS) from E. coli stimulated the production of C 3 by HUVEC. The effect of TNF-β, one of the most effective substances to stimulate C 3 production by HUVEC, reached plateau at the concentration of 200 U/ml. The production of C 3 decreased significantly after 4 passages of cell strains established from the human umbilical cord vein.
The relationship between inflammation and complement production by HUVEC is now in investigation.
