Abstract
A new cyclosporin A (CSA) ointment was prepared and its effect on contact skin reactions was evaluated in guinea pigs. 0.1% or 0.5% dinitrofluorobenzene (DNFB) -induced contact skin reactions were inhibited by twice daily topical application of 1-10% CSA ointment preparation, which showed more potency as compared to those of other topical CSA's or steroids. In contrast to oral administration of CSA (50 mg/kg) for 4 days, topical application over the same period resulted in more suppressive effect on contact skin reactions with rather lower CSA levels in blood (150 ng/ml) . CSA contents of this preparation were little changed even at 60°C for 3 months.
These results indicate that this new CSA ointment is effective and stable preparation. Moreover, there would be a possibility that this preparation may be valuable in the treatment of human cutaneous immune-mediated disorders.
