Changes in active oxygen generation by acidic non-steroidal anti-inflammatory drugs

Abstract

Effect of acidic non-steroidal anti-inflammatory drugs (NSAIDs), indomethacin, floctafenine and tiaprofenic acid, on active oxygen generation from guinea pig peritoneal (GPtPMN) and human peripheral neutrophils (HPPMN) were studied. NSAIDs suppressed formylmethionyl-leucyl-phenylalanine (FM LP) -induced active oxygen generation from GPtPMN.
Granulocyte colony-stimulating factor (G-CSF) enhanced receptor-mediated O^-₂ generation. Such a priming effect was closely correlated with proteintyrosyl phosphorylation of specific HPPMN proteins. NSAIDs also suppressed both the priming effect and the protein-tyrosyl phosphorylation by G-CSF in concentration dependent manners, and the effective concentrations were lower than those for the active oxigen generation from GPtPMN.
These results suggest that NSAIDs effect not only an activation step (s) of NADPH-oxidase but also an early step (s) of neutrophils activation.