Abstract
We used flow cytometry to investigate the function and the surface membrane protein expression by platelet derived microparticles from normal individuals. Microparticles were detected by both forward scatter and side scatter using flow cytometry. The binding of coagulation factor on microparticles was investigated by using monoclonal anti Factor X (Xa) antibody. Microparticles released from platelets after activation with the calcium ionophore A23187 and the binding of Factor Xa to microparticles increased markedly. These microparticles contained not only glycoprotein IIb/IIIa or I b but also P-selectin. On the other hand, microparticles were also released by the activation of complement system. Furthermore, these releases increased by the incubation with normal leukocytes.
Our findings suggest that platelet-derived microparticles participate the inflammatory thrombosis and hemostasis.
