Abstract
We measured levels of 11-dehydro-thromboxane B2 (11DT) and 2, 3-dinor-6-ketoprostaglandin F1α (23D6) in samples of urine collected at rest early in the morning from 72 normal children (36 boys and 36 girls), ranging in age from 1 to 18 years. Prostaglandins (PGs) were extracted from acidified sample by octadecylsilyl silica (Fuji Gel, Tokyo) suspension. After deproteinization and defattation were performed, PGs were eluted by ethylacetate. Then the dried residue containing PGs were fractionated by silica minicolumn BOND ELUT Si (Varian, Harbor City, Calif.) . We assayed PGs using RIA method.
No significant sex-related differences were observed in urinary 11DT or 23D6 levels. Urinary levels of 11DT and 23D6 were significantly high during the latter half of infancy (between 1 and 3 years after birth), and they tended to decrease gradually with age thereafter, without showing any further particularly marked change until puberty.
11DT and 23D6 are major metabolites of thromboxane A2 (TXA2) and prostaglandin I2 (PGI2) eliminated by way of urine, respectively. Their levels in urine are promising as indicators of systemic TXA2 and PGI2 production and replace the place of plasma prostanoid levels, a less reliable indicator conventionally used.
