Abstract
We developed a method for the simultaneous estimation of antigenspecific IgE and IgG antibodies in the serum of rats sensitized with DNP-As. An IgE capture ELISA employing rabbit antibodies against monoclonal DNP-specific rat IgE was applied to estimate the rat IgE antibodies to DNP-As. IgE antibodies in the serum of rats sensitized with DNP-As were first detected at day 6. The IgE antibody level increased to its highest level on day 8 during the course of sensitization with DNP-As, and was sharply decreased on days 20-25.On the other hand, the IgG antibody level increased to its highest level on day 10 during the course of sensitization with DNP-As, and decreased gradually on days 15-25. From the above-mentioned results, it is shown that the elevation of the antigen-specific IgG antibody level decreases the antigen-specific IgE antibody level in rats sensitized with DNP-As.
