Abstract
Several attempts have been already made in our laboratory to determine the order of appearance of various macrophages (Mφ) in the peritoneal cavity or spleen of mice after intraperitoneal (ip) or intravenous (iv) administration of various immunostimulants. Studies to discriminate Mφ of different function were conducted by a newly proposed analysis on their Fcγ receptor (FcγR) expressions : the expression of FcγRs on mouse Mφs obtained by various stimulants were examined by a newly-proposed luminol-dependent chemiluminescence analysis, where different subclass IgGs against hapten (TNP-SRBC) were used for the elicitation of the chemiluminescence from Mφ.
When a live Listeria organism was ip given or killed Corynebacterium parvum was given by iv injection, the appearance of both FcR I and FcR II on day 5 (Listeria), and on day 7 (Corynebacterium) was observed. The former Mφ, which were endowed with listericidal activity, were accompanied by a relatively dominant FcR I expression and the latter, endowed with the lowered Con A induced DNA synthesis, were accompanied by the relatively dominant FcR II expression.
Distinctive discrimination of the effector cells and the suppressr cells on FcγR exprssion was obtained after in vitro or in vivo treatments of inflammatory Mφs with various agents. In vivo and in vitro administration of γIFNαA/D resulted in the appearance of only FcR I on resident peritoneal Mφ. On the other hand, in vivo administration of TAP (immunosuppressive acidic protein) resulted in the appearance of FcR II on spleen Mφ, in parallel with the appearance of suppressor activity on Con A induced DNA synthesis of lymphocytes. Thus it was found that the effector cells tended to express FcR I and the suppressor cells, FcR II.
