Abstract
The purpose of this study was to determine the effect of plasma fibronectin (P-FN) on neutrophil function. For this purpose, the interaction of P-FN with neutrophil was examined in two ways. Neutrophils were obtained from human peripheral blood, and particle binding and ingestion were estimated in reaction mixtures with or without P-FN. (a) Particle binding was ascertained by the rosette-forming method using EA or EAC as indicator cells. (b) Percent ingestion of fluorescent microspheres was assayed by flow cytometry.
The results showed that neither particle binding nor ingestion was promoted by the presence of P-FN in reaction mixtures with test particles. From these results, it is suggested that P-FN may not serve as an opsonin to neutrophils.
