Abstract
In order to clarify the role of entothelial cell in systemic vascular diseases such as arteriosclerosis, diabetic microangiopathy and vasculitis of collagen diseases, the ability of human umbilical endothelial cell cultured for overnight in serum free media RITC 80-7 in MEM to generate superoxide was investigated by using cytochrome C reduction method. It was found that human endothelial cell cultured for overnight was producing spontaneously half amount of superoxide of human monocyte derived macrophage and was producing with PMA one twelfth amount of superoxide of human monocyte derived macrophage. The mechanism of endothelial cell to generate superoxide are remained unsolved.
