Abstract
A monoclonal antibody has been produced in the mouse system using copper-zinc superoxide dismutase (Cu, Zn-SOD) isolated from human blood. According to ELISA, the antibody was of the immunoglobulin Gl class and slightly cross-reacted with bovine and canine Cu, Zn-SOD but not with human serum albumin, α-globulin β-globulin and γ-globulin. Immunohistochemical analysis showed the localization of Cu, Zn-SOD in the hepatocytes, gastric glandular cells, colonic absorptive epithelial cells, ciliated bronchiolar cells, cells lining renal tubule in the nephron, Langerhans islet and exocrine centro acinus cells in the pancreas and neurons of the central nervous system.
