Effect of Syosaiko-to on the metabolism of arachidonic acid

Abstract

When a macrophage was stimulated with fMet-Leu-Phe, arachidonic acid was released. The release of arachidonic acid was inhibited by the treatment of Syosaiko-to, dose-dependently. And the soluble fraction separated from macrophages that were treated with 100μg/ml of Syosaiko-to inhibited the release of [¹⁴C] -arachidonic acid resulting from the incubation with porcine pancreatic phospholipase A2 and α-parmitoyl-β- [¹⁴C] -arachidonyl phosphatidylcholine. From these findings, it was suggested that Syosaiko-to had an inhibitory effect on arachidonic cascade and this effect was due to the induction of phospholipase A2 inhibitory protein.