Abstract
We studied assay system of simultaneous extraction of thromboxane B2 (TXB2), 6-keto-prostaglandin F1α (6KF1) and prostaglandin E2 (PGE2) . Accordingly, TXB2, 6KF1 and PGE2 were fractionated by simultaneous extraction system using octadecylsilyl silica (ODS) suspention and silica minicolumn (BOND ELUT Si) . These fractionated substances were measuerd by radioimmunoassay (RIA) .
Concentration obtained by this assay system in plasma sample of normal individuals (male 47, female 60) were TXB2: 14-50pg/ml, 6KF1: 12-33pg/ml and PGE2: 8.4pg/ ml (male), 4.4pg/ml (female) respectively.
Therefore, we concluded that measurements of TXB2, 6KF1 and PGE2 by our assay system were superior in smaller amount of sample simpler procedures resulting in mass specimen handling and easy for clinical application compared to other assay systems.
