Abstract
Thromboxane (TX) B2 is formed by platelets during the isolation of blood plasma. The determined level of TXB2 in plasma sample does not reflect the quantitative endogenous production of TXA2. Because of this complication, it is more suitable to determine the level of 11-dehydro-TXB2, one of the most prominent metabolites of TXB2 which is not formed in the blood, for the indication of TXA2 synthesis. Therefore, we attempted to develop a sensitive and reproducible enzymeimmunoassay for measuring 11-dehydro-TXB2. TXB2, as heterologous hapten, was coupled with β-D-galactosidase using γ-aminobutylic acid as a spacer. Using this method, measurement of 11-dehydro-TXB2 within the range of 25-50000 picograms per tube was possible. Crossreactivities for TXB2, 2, 3-dinor-TXB2 were 9.3% and 3.8%, respectively. However, the crossreactivity for other prostanoids tested was 0.3%.
