Abstract
The mitogenic activity of TJ-48 in murine lymphoid cells was examined to get a clue for the augmentation of antibody production by TJ-48.
Mitogenic activity of TJ-48 was detected in spleen cells, lymphonodus cells but not in thymocytes. The effect was abolished by pretreatment with anti-Ig antibody but not affected by pretreatment with anti-Thy 1.2 antibody. Coculture of spleen cells with TJ-48 resulted in increased number of sIgM-, sIgG- or sIgD-positive cells according to FAGS analysis. The increase in mitosis by TJ-48 was abolished by the elimination of adherent cells and the readdition of adherent cells recovered the effect of TJ-48. These data indicate that TJ-48 is a B cell mitogen and the activity is T cell-independent and adherent cell-dependent. The mitogenic activity of TJ-48 may account for the augmentation of the immune response.
