2017 Volume 32 Pages 13-20
【Background】
Feto-maternal tolerance by regulatory T cells (Treg) is an important system. We have reported that paternal antigen specific Treg was increased after the priming of seminal fluid in allogeneic mating mice. Antigen specific regulatory T cells are induced after antigen presentation by tolerogenic dendritic cells (DC). Here, we examined phenotype and function of the uterine DCs in mice .
【Method】
(1)Female BALB/c mice were mated with male DBA/2 mice (allogeneic mating) , and we analyzed the surface marker of DCs by the flow cytometry. To analyze the role of seminal fluid in DCs, we prepared seminal vesicle resected male DBA/2 mice (SVX). Female BALB/c mice were mated with male DBA/2 mice (SVX).
(2)To analyze of DCs function, mixed lymphoid reaction assay was performed.
【Result】
(1)CD86 and MHC class IIexpressions on DCs were significantly decreased in uterus on day3.5 pc and day5.5 pc in allogeneic mating. On the other hand, B7-DC expressions on DCs were significantly increased in uterus on day 3.5 pc and day5.5 pc in allogeneic mating. These molecules on DCs did not change in SVX allogeneic mating.
(2)DCs derived from uterus in allogeneic mating suppressed the proliferation of female spelenocytes, but DCs derived from uterus in SVX allogeneic mating did not suppress the proliferation.
【Conclusion】
Seminal fluid priming is important for the induction of tolerogenic DCs in allogeneic mating mice.
