Abstract
The developmental competence in vitro of immature oocytes from mouse early preantral follicles in a simplified culture was investigated. The follicles ware obtained from 4-10 weeks and 14 days old mice, respectively. On day 12 of culture, most of cultured follicles ware survived (100% vs 97%) in this culture system. The granulosa cell outgrowth was observed in all of surviving follicles. The oocytes grown in vitro ware leaded to GVBD (84% vs 96%), and 44% of them reached Metaphase II, respectively. This results show that this culture system is valid in not only preantaral follicles from prepuberal mouse but also that of postpuberal periods.
