Abstract
Massive parallel pyrosequencing of 16S rRNA genes has allowed to profile bacterial community structure of gastro-intestinal (GI) -tract samples in detail. Critical points in this analytical method are to design a set of universal primers which is able to amplify the highly diversed 16S rRNA genes of GI-tract bacterial member and how to convert massive sequenced data into bacterial population data. In this review, we discuss the current status and problems in GI microbial composition analysis based pyrosequence.
