Abstract
Next-generation sequencing (NGS) technology is a fundamental means of studying genome, transcriptome, and microbiome. We can analyze NGS data of bacteria such as Lactobacillus using laptop computer nowadays. However, for many non-bioinformaticians, it is difficult to construct an environment for analyzing NGS data. In this review, we describe educational programs, websites, application softwares, programming languages, and webtools. An example of genome analysis of Lactobacillus casei 12A is also provided.