Abstract
Graphical user interface (GUI) is useful to perform general tasks. Analysis of next-generation sequencing (NGS) data is, however, non-trivial tasks. Many methods dedicated to NGS data have been implemented on Linux system thatprovides a command line interface (CLI) as an analysis environment. Therefore, it is desirable for researchers to analyze NGS data on Linux system. We here show the CLIs on Windows (i.e., Command prompts) and Macintosh (i.e., Terminal) systems in contrast with those GUIs. We also describe some basic commands such as “dir” and “ls.”