Abstract
Human peripheral monocytes could be shown to produce and secrete procoagulant and fibrinolytic factor. T-lymphocytes have been shown to collaboratively initiate or amplify monocyte procoagulant and fibrinolytic activity responses to the stimulation of LPS, con A and PHA.
The procoagulant activity has been identified as thromboplastin (tissue factor) by its sensitivity to phospholipase C and loss of activity in factor VII-and factor X-deficient plasma. Inducible thromboplastin generation would be most likely dependent on de novo synthesis of RNA, protein and metabolites of non-cyclooxygenase and non-lipoxygenase pathway in arachidonic acid cascade. Induction of monocyte procoagulant activity in collaboration with T-lymphocytes has required for the synthesis of protein and metabolites of arachidonic acid cascade in monocytes but not in T-lymphocytes.
Enzymography has demonstrsted that fibrinolytic factors from monocytes were urokinase-type plasminogen activator with molecular weight of 55KDa and contained small amount of 33KDa. Plasminogen activator inhibitor has been produced from monocytes concurrent with production of plasminogen activator. The fact that this urokinase-type plasminogen activator was not inhibited by plasminogen activator inhibitor from monocytes would mean this plasminogen activator to be single chain urokinase-type plasminogen activator.
