Abstract
In the previous papers, the author has drawn roughly the following conclusion after the investigation in the influences of various chemical agents on the acylase ; i.e., not infrequently, the action of acylase in the liver or in the kidney of rabbit is enhanced, and, on the other hand, a specific action of acylase newly becomes active, though it is weakly, when a rabbit is administered with various chemical agents. Furthermore, he also confirmed that the hydrolysis of substrate such as hippuric acid or cinnamoyl glycine and that of taurocholic acid does not necessarily run in paraljel each other, and that the substances which possess aminoradical in β position and possess SO3H-radical such as benzoylaminooxypropanesulphonic acid resist the extracts of liver or kidney very strongly, resulting no hydrolysis in all cases. The author also observed that urethane, which is frequently employed for animal experiments, is at least almost ideal anaesthetic in view of the intensity of its influence on the acylase.
At this point, interested by the question as to how the action of acylase of heart is affected by the administration of various chemical agents, the author performed the present experiments following the experiments described in his previous papers. The conclusions drawn from the present experiments are as follows.
1) The heart extract prepared from normal rabbit almost does. not decompose hippuric acid, or very slightly if it does. Cinnamoyl glycine is even more difficult to be decomposed, and taurocholic acid and glycocholic acid are not decomposed by it absolutely.
2) By the oral or subcutaneous administration of glycine, heart extract decomposes hippuric acid very readily ; and the hydrolysis of cinnamoyl glycine becomes evident. The hydrolysis of taurocholic acid is also promoted.
3) In the case of the subcutaneous injection of caffeine, the hydrolysis of hippuric acid is also promoted ; and the decomposition of cinnamoyl glycine also becomes evident. However, conjugated bile acid is not affected.
4) In the case of the oral administration of benzoic acid and in the cases of the subcutaneous administration of cinnamic acid or hippuric acid, only hippuric acid, or cinnamoyl glycine too, are very well decomposed.
5) In certain cases, the decomposition of cinnamoyl glycine is extremely well promoted by the oral administration of glycocholic acid.
6) In the case of single administration of cholic acid, glycocholic acid or taurocholic acid, the decomposition of conjugated bile acid is not evident in general. On the contrary, in the cases of combined administration of cholic acid and taurine or taurocholic acid and taurine, taurocholic acid is slightly decomposed.
7) In the case of urethane is administered, the hydrolysis of hippuric acid, cinnamoyl glycine and taurocholic acid remains the same as that of the control cases.
8) In the case of the administration of chloral hydrate, the decompositions of hippuric acid and cinnamoyl glycine are often promoted and both conjugated bile acids are also found decomposed.
9) In the case of the administration of chloroform, the decompositions of hippuric acid, cinnamoyl glycine and glycocholic acid become evident.
In short, when various chemical agents are administered to rabbits, it was learnt that the action of acylase of heart is often enhanced.
