Abstract
The author planned the present experiment in an attempt to clarify to certain extent the difference between saccharin and dulcin in their toxicity by tracing the fate of saccharin in the living subject for the comparative study with that of dulcin. As it was learnt that the embryo of the hen's egg, which received the injection of 66 mg of saccharin, develops very well and hatches out perfectly, and that, pathological histologically, no injuries are found in the liver of the embryo, plans were made afresh to investigate the behavior of the hepatic enzymes, especially of the proteolytic enzymes, for the enzymatic study of the toxicity of saccharin.
1) Most part ofd the saccharin injected into a developing hen's egg is rapidly excreted without decomposition into the allantoic fluid.
2) The hydrolysis to protein and diglycine by the hepatic enzymes contained in the liver of the embryo hatched out from the hen's egg, which has been injected with saccharin, does not demonstrate any remarkable difference from that of the embryo from the control egg without preliminary treatment.
In short, in view of the fact that saccharin injected into developing hen's egg is rapidly excreted without remarkable findings of pathological histological injuries in the kidney, as stated in the above, and that, pathological histologically as well as enzymatic chemically, saccharin induces almost the similar findings in the liver to those found in the control cases, the author considered that the non-toxicity of saccharin was proven.
