Abstract
It has been clarified by Tomosaburo Ogata and members of his school that salivary gland is secreting internally as well as externally. The salivary gland hormon was named “parotin.”
It has already been reported that parotin is very closely related to the development of bone and dental tissues, and that it is related to the calcium metabolism. It is also known by the microscopical and macroscopical investigations on the embryonal stage of animals that parotin in an adequate amount acts promotingly on the general development and the development of feathers of chick embryo, and that the development inhibitory action of calcium chloride on the chick embryo is eliminated by parotin.
In the present paper, the author attempted to trace from the pharmacological standpoint the correlationships between salivary gland hormon (parotin) and calcium employing fertilized eggs of white leghorn.
Eggs of white leghorn weighing approximately 50 g were selected. Solutions of, parotin were prepared with physiological saline to contain 0.2 mg, 0.01 mg, 0.1γ and 0.01γ of parotin per 0.1 cc of physiological saline. Eggs of each group received 0.1 cc of the white by usual tech-parotin solutions prepared as above respectively within the egg nique. Each egg of the control group was injected with 0.1 cc of physiological saline in a similar manner, and each egg of another group was left untreated. Eggs of the above S groups were incubated, and 5 eggs were taken out at random from each group after 9 days, ' 12 days' 15 days' and 18 days' incubation as samples. The calcium content of allantoic fluid the calcium content of the whole embryo and the amount of ash residue of each of these eggs thus taken out were estimated. As the results, the followings were obtained.
The calcium content in the allantoic fluid was found only minute after 9 days and 12 days. Futhermore, the results could not be compared due too large experimental error .in the determination technique.
After 15 days' incubation, a remarkable differences in the calcium content of the allantoic fluid, were recognized, showing a significant difference by statistical study. In the cases of the group which received a highly concentrated parotin solution (0.2 mg per 0.1 cc), a large amount of calcium was proved to be present, but no difference was recognized from the control group which received physiological saline. In the cases of the group which received parotin solution of low concentration (0.1γ and 0.01γ), the values were found smaller compared with those with solution of high concentration and with the control groups. In other words, the calcium content of the allantoic fluid vlas found decreased when an adequate amount of parotin was applied.
Due to scarcity in the calcium content in the whole embryo after 9 days' incubation, 5 eggs had to be tested at one time, thus making statistical analysis of the results impossible. However, an arythmetic average indicating decrease in the higher concentration and increase in the lower conceentration was obtained.
A statistically significant difference in the calcium content of the whole embryo way recognized after 12 days' incubation. The calcium content of the embryo received parotin solution of high concentration was found smaller than that of the physiological saline control group, and the value for the lower concentration group was found larger compared with that for the higher concentration group. But the increment in this case did not show any statistically significant difference with that for the physiological saline control group.
No statistically significant difference was recognized in the calcium content of the whole embryo after 15 dais' and 18 days' incubation. Considering from the arythmetic average, the low concentration group demonstrated the largest increment.
The ratio of the amount of ash residue to the calcium content of the whole embryo was investigated
