Abstract
Following the investigation by Yoshimatsu on the blood coagulation promoting substance and blood platelet increasing substance contained in the splenic component, the author attempted to conduct experiments on the crystaline substance and extracts isolated from the fraction, which is left untouched, of cattle spleen, and to clarify the presence of the fraction containing strong coagulation promoting substance by a similar method to Yoshimatsu's experiments. Further, the author prepared splenic extract of horse and cattle for his experiments by a different procedures from that hitherto adopted by the author's Department. The author further investigated the behavior of the blood coagulation promoting substance contained in the human spleen (patients suffering from Banti's disease, hemolytic jaundice, laryngeal tuberculosis and esophagus cancer) and compared the results with those from the spleen of animals. The results obtained are as follows.
1) Among the crystaline substances isolated from the 94 % alcohol insoluble fraction of cattle spleen, Crystal [I-R-1] and Crystal [II-R-1] have blood coagulation promoting activity and blood platelet increasing activity. Further, stronger activity is demonstrated when these two crystals are applied in combination, or when these two crystals combined with 94 % alcohol soluble extract of cattle spleen is applied. Crystal [I-R-M-2] possesses only weak activity.
2) Among extracts isolated from 94 % alcohol insoluble fraction of cattle spleen, [A] and [B] possess the above two activities, though they are weak, but [C] possesses almost no such activity.
3) These two activities of extract treated by cadmium sulfate of horse spleen are weak.
4) Activities of cattle spleen extract treated by acetone are stronger than those of horse spleen extract treated by cadmium sulfate.
5) The blood coagulation inhibiting factor exists in the spleen of patients suffering from Banti's disease or hemolytic jaundice (acetone insoluble extract of dehematized spleen) .
6) In the spleen of patients suffering from Banti's disease, anemia inducing factor is also contained.
7) The blood coagulation inhibiting factor and anemia inducing factor are heat stable, and their activities are not lost even after a long standing.
8) The blood coagulation inhibiting factor is not generally transfered to the blood.
9) In the spleen of patients suffering from laryngeal tuberculosis of from esophagus cancer (acetone insoluble extract of dehematized spleen), blood coagulation promoting factor can be proven to the same degree as in the normal spleen of various animals. This substance is transfered to blood to certain extent.
10) The activity of the blood coagulation promoting factor isolated from human spleen is far less active compared with that of Crystal [I-R-M-1] (C6H13O2N) isolated from 94 % alcohol soluble fraction of cattle spleen by Tsunoo et al.
11) It is presumed that the blood coagulation promoting factor is not necessarily single, but the activity is demonstrated by the synergistic action of certain number of such active factors.
In short, the author successfully isolated crystals or fractions possessing blood coagulation promoting activity from spleen of various animals, but their activities are found to be far less active when compared with that possessed by the Crystal [I-R-M-1] isolated by Tsunoo et al previously. Furthermore, the presence of the blood coagulation inhibiting factor in the spleen of patients suffering from Banti's disease or hemolytic jaundice was confirmed.
