STUDIES ON THE NUTRIENTS FOR THE TISSUE CULTURE OF HUMAN EPIDERMIS

I. pH of the nutrients and its effect upon the epidermal cell proliferation on tissue culture

Abstract

As a part of the studies on the nutrients for the tissue culture of epidermal cells, the author investigated the influence of the each constituent of nutrient and its pH with special emphasis on the influence for the tissue culture of human epidermis.
1, pH of human serum collected under usual condition without being air-tight was 8.0±0.1
2. pH of the chick embryo extract diluted with the same quantity of Ringer's solution was 6.5±0.06.
3. pH of the nutrient composed of salt solution (Ringer), human serm and chickembryo extract in a ratio of 4 : 4 : 2 was 7.8±0.08.
4. Nutrient adjusted of its pH by the addition of N/10 HCl and N/10 KH₂PO₄ practically showed no change in provided the nutrient was hermetically sealed. While, when. CO₂ gas was used, the pH of nutrient showed a tendency to increase.
5. The nutrient containing N/10 HCl and N/10 KH₂PO₄ in a concentration of 10% inhibited the membraneous outgrowth of the epitherial cell. Increase in the concentration.of CO₂ gas, likewise, inhibited the membraneous outgrowth of the epitherial cell.
6. Epitherial cell showed poor proliferation in the nutrient of high pH concentration This poor growth of epitherial cell might also due to the toxic charactor of HCI, KH₂PO₄. or CO₂ gas used for the adjustment of pH.