Abstract
Employing a total of 64 rabbits, tubed pedicles of the same size (10 × 5 cm) were made on the dorsal surface of both left and right by the method of Davis and Kitlowski. Tubed pedicles on the right side received Histamin iontophoresis, injection of Histamin solution, Trazolin preparation (Imidain), Kalikrein, protein assimilating hormone Norandrostenoron phenylpropyonate (Durabolin), general aminoacid preparation (Moriamin), application of Hirudoid ointment, or the iraadiation of radiothermy. Separately, as controls, tubed pedicles were made in the same manner and the right side pedicles received the injection of mere physiological saline in one group, daily massage for 10 minutes in another group and no treatment in the third group. Animals were sacrificed by sending air through the ear vein and after thoracotomy a contrast medium (50-60 cc of Hypec or 76% Urographin) was sent into the pedicle throgh the aorta descendens for vascography. In addition, parrafin and corrosion samples were prepared from a part of the tubed pedicle for the observation of the vascular augmentation in each tubed pedicle. Stated in the following are the results obtained thereof :
1. When Histamin iontophoresis was applid the most remarkable vascular augmentation was observed, Injection of protein assimilating hormone Durabolin, Kallikrein and the applicatiod of Hirudoid ointment which showed also relatively remarkable augmentation.
2. In the animals received the injection of protein assimlating hormone (Durabolin) and Kallikrein, some vascular augmentation was observed on the tubed pedicle of the side without injection which is considered to by due to the systemic effect of hormonal preparations. It appears, however, these preparations have some direct topical effect aside from the systemic one as there was recognized still more remarkable vascular augmentation on the tubed pedicle of the side received injection.
3. Tubed pedicles prepared after application of Histamin iontophoresis as preliminary treatment also showed some vascular augmentation but it was of very mild one.
4. Contrary to the author's expectation, animals received the injection of Histamin solution, Trazolin preparation (Imidalin), general aminoacid preparation (Moriamin) and the irradiation of radiothermy showed virtually no vascular augmentation on their vascograms nor in their histological findings. It is too early, howeve, to draw any conclusions from these results on account of the experimental conditions such as the period of experiment employed in the present stuy.
5. It is difficult to expect the same clinical effects on human beings because of a considerable difference in the construction of skin between rabbits and human beings, for and instance, rabbits do not possess sweat glands. But the results mentioned above may be considered to show the general tendency of respective treatment.
6. Accordingly, the results of present study are considered to offer a number of interesting problems as to the clinical application of Histamin iontophoresis, protein assimilating hormone injection (Durabolin), Kallikrein injection and the application of Hirudoid ointment to the skin grafting.
