Abstract
This study was undertaken to assess the effect of rheumtaoid factor like substance (RFLS) on adjuvant arthritis, and the assay of serum antibodies and histological examinations of various organs were also carried out.
The adjuvant which contained 6 mg/ml dry-killed mycobacterium tuberculosis (Type H-2) in parafin oil was used in this study, and was inoculated at the rate of 0.1 ml intradermally into the left hind footpad. The animals used were female, 2-3 month old Sprangue Dawley rats. For production of RFLS, rats of the two groups were treated with isologous denatured γG and BSA-anti BSA complexes at various times before the adjuvant injection. Rats of the other groups received BSA at various times before adjuvant injection or a single challenge injection of adjuvant.
The remarkable inhibition of the arthritis was observed in the group of the rats which were given simultaneouse injection of BSA and adjuvant, in comparison with control rats which were given adjuvant alone.
The similar inhibition was observed in the rats which received the pretreatment with the denatured γG and the complexes 5 weeks, 3 weeks and 1 week before, and in the rats which received the same pretreatment 2 weeks and 1 week before adjuvant injection.
The histological fidings of hearts, lungs, livers, kidneys and spleens were demonstroted no particular differences between the groups of rats with and without arthritis.
