Abstract
The change of serum insulin level after intravenous glucose loading in dogs was studied, regarding especially to comparison between immunoreactive insulin (IRI) with and without acid-ethanol extraction in the femoral, hepatic and portal vein.
The conclusions were as follows
1) By acid-ethanol extraction IRI was still detectable in the sera after free insulin was removed from the sera by gel-filtration.
2) Acid-ethanol extracted component of serum behaved immunologically similar to crystalline insulin.
3) IRI changed parallet to blood glucose level in the femoral, hepatic and portal vein after glucose loading. IRI and extracted IRI, however, exhibited a reciprocal relationship in the portal vein, but not in the femoral and hepatic vein.
4) It is not cerr tamn on the role of the circulating extractable IRI and also the rela-tion between extractable IRI and NSILA, proinsulsn or bioginsulin.
