Abstract
In this paper, we report about the keratinase of Microsporum canis. The isolation and purification method was as follows: at first culture broth was filtrated, and then passed through a DEAE Cellulose. The effluent adjusted to pH 6.1, was fixed with a CM Cellulose, eluted with 0.1 M NaCl, and passed again through a DEAE Cellulose. Finally the material was applied to Sephadex G 75. The sample was divided into two Isozyme. The molecular weight of keratinase is about 32, 000 and 20, 000. The keratinase A and B have a pH optimum of 7.5, their optimum temperature is at 47°C, optimum ionic strength is at 0.01 M NaCl in 28 mM phosphate buffer, pH 7.8. Disc electrophoresis was carried out according to the method of Yutaka Nagai. The keratinase A and B each gave a single band on 10% gel run at pH 4.0. These result is differed from the keratinase of Microsporum gypseum and Trichophyton mentagrophytes.
