Abstract
Mitogenic activity of DNP-Ficoll to SRBC-primed spleen cells (C57BL/6 and ICR mice) was investigated in microculture without FBS by determining3H-thymidine incorporation into acid-insoluble fragment from their cells.
The spleen cell suspensions were cultured for 48hr at 37°C after addition of this TI-2 antigen and3H-thymidine. DNP-Ficoll was added to 200-μl culture at a ratio of 0.0002, 0.002 or 0.02μ1/ml. When the spleens excised from C57BL/6 mice 6 days after immunization with SRBC antigen (4×108cells/mouse, i.v.) were used, enhancement of radioactivity was observed in the DNP-Ficoll treated cultures. This enhancement was again found in ICR mouse spleen cells (6-day nostimmunization) .
However, DNA syntheses of T or B lymphocytes separated from the spleen cell suspension by nylon wool column were not stimulated in the presence of DNP-Ficoll.
