Abstract
The purpose of this study is to clarify the effect of 1α-OH-D3on the bone metabolism through the process of the bone graft. Eighty seven male rats of 7 week-old were grafted on the middle of femur from tibial bone and were given 1α-OH-D3every day. Following biochemical changes in the serum, radiological findings and histological findings of bone tissue were studied. The results are summarized as follows: (1) Serum calcium in the 1α-OH-D3group was noted at high level, but it was in normal ranges. 1α-OH-D3elavated the serum level of alkaline phosphatase activity at the early stage. (2) At the early stage of the bone graft, bone resorption in the 1α-OH-D3group could be seen severely around the grafted bone through the radiological findings and contact microradiograms, and also the bone formation could be observed amply from the grafted bone to endosteal area of femur. (3) Through the histological findings, 1α-OH-D3could stimulate osteoclasts and osteoblasts and promote the differentiation and function of them. In conclusion, the administration of 1α-OH-D3during the process of the long bone graft is useful in stimulating the direct bone formation.
