EFFECT OF ALMINOPROFEN ON PRIMARY CULTURES OF HEPATOCYTES AND ERYTHROCYTES IN RAT

Abstract

It was investigated that effects of alminoprofen, as an analgesic anti-inflammatory drug, on the enzyme leakage and¹⁴C-leucine uptake in primary cultures of rat hepatocytes, and on the hemolysis in rat erythrocytes. In primary culture of rat hepatocytes, carbon tetrachloride (CCl₄) increased significantly the leakages of GOT, GPT, LDH and OCT into the medium. However, the leakages of GOT and OCT were inhibited by alminoprofen, as well as phenylpropionic acid derivatives, ibuprofen, ketoprofen and flurbiprofen. Also, alminoprofen and ibuprofen (1×10^-6-1×10^-3M) did not affect on the¹⁴C-leucine uptake into the protein. And, CCl₄at the concentration of 1×10^-2M caused the significant decrease of¹⁴C-leucine uptake into the protein in primary culture of rat hepatocytes. Furthermore, alminoprofen showed a membrane stabilizing effect on the hypotonic hemolysis using the rat erythrocytes, similar to ibuprofen, ketoprofen and flurbiprofen. From these results, it seems that alminoprofen, ibuprofen, ketoprofen and flurbiprofen do not have a direct damaging effect on hepatocytes.