EFFECT OF SCHIZOPHYLLAN AND OTHER IMMUNOSTIMULANTS ON PHAGOCYTIC ACTIVITY OF MACROPHAGE CELL LINE
1986 Volume 46 Issue 3 Pages 391-397
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1986 Volume 46 Issue 3 Pages 391-397
Effect of schizophyllan (SPG), a simple glucan produced bySchizophyllumcommune, and other immunostimulants on candidastatic activity of murine macrophage cell line J774.1 was examinedin vitro. The J774.1 cells treated with each immunostimulant were seeded in 96-multiwell tissue culture plates and infected with serially (2-fold) dilutedCandida parapsilosiscells. Fungistatic index (FI) was estimated after 24 hours incubation. (1) When J774.1 cells were cultivated in the presence of SPG at the concentrations of 10-4-102μg/ml, the candidastatic activity proved to be augmented, showing diphasic pattern. The first peak was observed at one hour cultivation (FI: 2.5-9.0), and the second between 24 and 72 hours (FI: 2.12-7.25) . (2) In the case of LPS, increase of candidastatic activity (FI: 4-8) was recognized after one hour cultivation at the concentrations of 10-5-10-1μg/ml, and maintained for 24 hours. (3) The activation of macrophage with krestin (FI: 4-32) could be observed at the higher concentrations (10-500μg/ml) . (4) Lentinan showed weak activation (FI: 2-4) at the concentrations of 10-1-10-3μg/ml after 24 hours cultivation. (5) Corynebacterium parvum, BCG-CWS and picibanil activated J774.1 cells at the very narrow range of concentrations after 48 hours cultivation. (6) No enhancement of candidastatic activity could be detected in the cases of lipid A, MDP, MDP-Lys (L18), DMG and levamisole.
