Abstract
Seaprose S (SAP), a proteinase clinically used as an anti-inflammatory agent, was administered intravenously to male Sprague-Dawley strain rats at a dosage of 4 mg/kg. Changes in kallikrein-kinin, coagulation and fibrinolysis systems were studied at 1, 3, 24 and 72 hours after injection. High molecular weight kininogen (HMW-kininogen) level was markedly decreased at 1 and 3 hours to trace levels. However, there were no differences in low molecular weight kininogen (LMW-kininogen) level, prekallikrein activity, and kininase II activity between the SAP-treated group and control (saline injected) group. The consumption of coagulation factors was suggested by a decrease in fibrinogen level and factor XIII activity, and prolongation of prothrombin time (PT) and partial thromboplastin time (PTT) . Plasma protein inhibitors, α2-plasmin inhibitor and antithrombin III activity decreased while plasminogen level remained unchanged. However, these changes of coagulation and the fibrinolysis system were still within normal limits. The results suggest that a marked decrease in HMW-kininogen is most probably attributable to one of the anti-inflammatory actions of SAP.
