Abstract
Lipopolysaccharide (LPS) was extracted from Campylobacter jejuni C1strain (C. jejuni) with phenol-water method. It was shown that molecular weight of C. jejuni LPS was lower than that of control LPS (E. coli and S. enteritidis) by SDS-polyacrylamide gel electrophoresis. The sugar components in C. jejuni LPS were glucose, galactose, heptose, glucosamine, pho-sphorylethanolamine, 4-amino-L-arabinose and 2-keto-3-deoxy-octonate. The fatty acid components were identified mainly 3-hydroxy-miristic acid (3-OH-14: 0) and palmitic acid (16: 0) ; miristic acid (14: 0) was trace amount. Biological activity of the LPS was tested on gelatinized limulus amoebocyte lysate, lethality on mouse, the local Shwartzman reaction and pyrogenicity in rabbit. It was shown that C. jejuni LPS was equal in biological activity to control LPS. C. jejuni LPS 100μg was given to mice, and the edema in small intestine, the single necrosis of hepatocytes and necrosis of the white pulps in the spleen microscopically were observed 4 days after administration. Immunological activity of the LPS were tested on mice spleen plaque forming cell (PFC) numbers and superoxide anion production of peritoneal macrophage, and the LPS (10μg) showed adjuvant effect on mice and enhancement of superoxide anion production in macrophage.
