Abstract
We studied the effects of SaSa senanesis Rehder extract (SE) and some crude drugs on membranes. SE was extracted from SaSa senanesis Rehder by alkaline hydrolysis, and six fractions (Fr. 1-Fr. 6) were obtained by silica gel column chromatography. SE protected erythrocytes from hypotonic hemolysis, and almost the same degree of protection was provided by Fr. 3 and Fr. 5. High concentrations of SE, Fr. 3 and Fr. 5 reduced surface tension. Leakage of GOT and GPT from the cultured hepatocytes was significantly decreased by SE. However, no fraction depressed the phase-transition temperature of the DPPC liposome bilayer, indicating that SE does not influence the fluidity of the phospholipid bilayer membrane. Inhibition of enzyme leakage from hepatocytes by SE was more potent than that of any other crude drug. These present results indicate that SE, composed of polysaccharide, lignin and chlorophyll, remarkably inhibits hypotonic hemolysis and enzyme leakage from cultured hepatocytes, and has a potent membrane protective effect.
