Abstract
The liver tissue pH was measured for the purpose of evaluate on of the viability of transplanted liver easily and early. [Materials and Methods] Male W istar strain rats weight 250g were used. The liver was removed by Kamada's method with necessary modifications. (Experiment 1) The tissue pH was measured at perfusion rates of: Group I, 0.05; Group II, 0.5; and Group III, 16 (ml/h/BWg) . (Experiment 2) The tissue pH preservated at 4°C and normal room temperature and ΔpH×preservation time were measured to calculate the 7-day survival rate. (Experiment 3) The tissue pH was measured in relation to various solutions for preservation-Group H: Hartman D solution, UW solution and Group UW, Group HP: Prostaglandin E1 (hereafter referrd to as PG) -added Hartman D solution (1μg/ml), and the 7-day survival rate after preserving for 6 hours was calculated. (Result) (Experiment 1) The fall in the tissue pH tended to be high in the order of Group II, III and I. (Experiment 2) Changes in the pH were hardly evident at normal room temperature. Normal room temperature pH reflected the survival rate and correlated with the value expressed in ΔpH×preservation time (min) . On the basis of this finding, the preservation time enabling 80% survival in Experiment 1 was estimated at Group I 150 minutes, Group II 540 minutes and Group III 135 minutes, showing the time in Group II to be the best preservation time to enable preservation for many hours. (Experiment 3) A sharp fall in pH to the minus side was seen from the beginning in Group H, while in Group UW a sharp increase in pH to the plus side followed by a slow decrese was noted, but the decrease did not reach the minus side. Group HP showed changes similar to those in Group UW. (Conclusion) Measuring the liver tissue pH over time in the simple immersion preservation of the liver was of use for evaluating viability easily and early, the survival rate could be predicted by the tissue pH×preservation time (min), and the value calculated by the 80 % survival rate was 4.86. The most suitable condition of preservation was perfusion rate of 0.5 ml/h/BWg, temperature at 4°C and the UW solution. It was conjectured that addition of PG to solution protected the liver.
