Abstract
Two kinds of cell lines, SASY-4 and SASY-1, were isolated from osteosarcoma and chondrosarcoma, respectively. The characteristics of these cell lines were analyzed and compared with those of other human osteosarcoma cell lines (Sans-2, Mg63, HOS, YR-2) and a human fibrosacroma cell line (HT1080) . Saos-2, YR-2, SASY-4, had numerous alkaline phosphatase (ALP) -positive cells, and SASY-1 had few ALP-positive cells. There were few ALP-positive cells in Mg63, HOS and HT1080. ALP activity was highest in enzymes and Saos-2, YR-2, SASY-4, and SASY-1 followed in that order. Enzyme activity was extremely low in Mg63, HOS, and in HT1080. Treatment of the cells with parathyroidhormone (PTH) induced a marked increase in cAMP production in YR-2 and Saos-2, and a slight increase in SASY-1 and SASY-4. There was no cAMP response by PTH stimulation in Mg63, HOS or HT1080. Immunohistochemical staining demonstrated that all cell lines produced type I collagen, whereas only SASY-1 cells produced type II collagen. Radioimmunoassay revealed that all cell lines produced undetectable levels of osteocalcin in the culture media under conventional culture conditions. After treatment of the cells with 1, 25 (OH) 2D3, only Mg63 cells produced osteocalcin. The results indicate that expression levels of osteoblastic phenotypes vary among the various human osteosarcomas. Production of type II collagen may serve as a simple but convenient marker for distinguishing chondrosarcoma cells from osteosarcoma cells.
