CELL CULTURE OF HUMAN SKIN CARCINOMA CELL LINES ON POST-MITOTIC HUMAN DERMAL FIBROBLAST (HDF) AS A FEEDER LAYER

Abstract

We established and maintained two kinds of human skin carcinoma cell lines; SSCC-1 from a cutaneous squamous cell carcinoma and SSCC-2 from the skin metastasis of an oral squamous cell carcinoma. Two cell lines were cultured with mitomycin C-treated post-mitotic human dermal fibroblasts (HDF) which served as the feeder layer. HDF facilitated the growth of SSCC-1 and 2, preferentially in low seeding densities. The speed of growth in SSCC-1 tended to parallel the density of HDF. The pattern of growth in SSCC-1 differed from that in SSCC-2. We speculated that the difference might be reflected in the nature of each cell line.