Abstract
We made a semi-automatic measurement system using a personal computer in which centromeres of specific chromosomes in human tumor cells were labeled by the fluorescence in situ hybridization (FISH), and the number of hybridized parts per nucleus was counted. In order to improve the precision of measurement, as basic data, the measurement was conducted to X chromosomes of normal lymphocytes. We propose a method to set the practical value of distance between adjacent spots; the spots closer than this distance should be considered to one spot.
