Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Regular Paper
Identification and in situ Detection of Two Lineages of Bacteroidales Ectosymbionts Associated with a Termite Gut Protist, Oxymonas sp.
Satoko NodaMiho KawaiHideaki NakajimaToshiaki KudoMoriya Ohkuma
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2006 Volume 21 Issue 1 Pages 16-22

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Abstract

Bacterial attachments often cover the entire surface of flagellated protists in the guts of termites. Based on PCR-amplified 16S rRNA gene sequences, we investigated the phylogenetic positions of the rod-shaped bacteria (ectosymbionts) attached to the protist Oxymonas sp. in the termite Neotermes koshunensis. Two distinct and unique lineages of the ectosymbionts within the order Bacteroidales were identified, each belonging to a cluster exclusively comprised of the sequences from termite gut. We designed two oligonucleotide probes specific for the two lineages, and successfully detected the ectosymbionts, each of which distributed over the entire surface of Oxymonas sp. However, few cells of Oxymonas sp. simultaneously harbored both lineages of the ectosymbionts.

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© Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology
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