Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Short Communication
A Novel Organophosphorus Pesticide Hydrolase Gene Encoded on a Plasmid in Burkholderia sp. Strain NF100
Kanako TagoSumiko YonezawaToshihide OhkouchiTomoyuki NinomiyaMasayuki HashimotoMasahito Hayatsu
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2006 Volume 21 Issue 1 Pages 53-57

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Abstract

The fenitrothion hydrolase gene encoded on the plasmid pNF2 in Burkholderia sp. strain NF100 was cloned and sequenced. The 6.5-kbp EcoRI fragment contained three ORFs (ORF1 to 3) the deduced amino acid sequences of which shared no significant homology with any sequence present in databases. The results of frameshift mutation showed that ORF1 and ORF2 were essential for full enzymatic activity, and ORF3 was not involved in the degradation of fenitrothion. Fenitrothion hydrolase was located in the cell membrane fraction. The DNA region containing the fenitrothion hydrolase gene was spontaneously lost from pNF2.

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© Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology
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